Li Ye, PhD
Professor and HHMI Investigator
Scripps Research Institute
Host(s): Kapil Ramachandran (Faculty)
Deciphering spatial and cellular targets of drug actions in the CNS
Recent advances in chemical, molecular, and genetic approaches have provided us with an unprecedented capacity to identify drug-target interactions. Meanwhile, rapid developments in single-cell and spatial omics technologies are revolutionizing our understanding of the molecular architecture of CNS. However, a significant gap remains in aligning our traditional understanding of drug actions, based on molecular affinities, with the in vivo cellular and spatial tissue heterogeneity revealed by these newer techniques. Recently, we developed technologies enabling high-resolution, multiplexable in situ small molecule drug imaging (clearing-assisted tissue click chemistry or CATCH). CATCH permits specific and robust in situ fluorescence imaging of target-bound drug molecules at subcellular resolution and enables the identification of target cell types in the mammalian brain. We foresee the integration of single-cell and spatial omics platforms, data, and concepts into the future framework for defining and understanding in vivo drug-target interactions and mechanisms of action.
Relevant Publications:
In situ identification of cellular drug targets in mammalian tissue
Venue Information:
Speaker Location: Jerome L. Greene Science Center, Kavli Auditorium, 9th Floor Lecture Hall
The Columbia Neuroscience Seminars have been organized to help build community and collaboration among researchers interested in this broad field across campus. The in-person activities, including the talks, provide meaningful interactions for the speakers, many of whom have traveled a long way to visit Columbia. However, if you are a Columbia researcher on another campus and are unable to attend the talk at the Jerome L. Greene Science Center, please email [email protected] at least 48 hours in advance to request an individual, one-time Zoom link (livestream only, no Q&A).
If you have a short- or long-term accommodation request (medical issue, travel, other concerns, etc.), or any other questions, please also reach out to [email protected].
Tuesdays@10 is a signature Zuckerman Institute initiative that aims to expose researchers at all levels to high-quality science and stimulate scientific discourse. The speakers featured in this series represent various fields and techniques in neuroscience, and include invited guests of the Columbia Neuroscience Seminars, the Zuckerman Institute's Local Circuits Affiliates Program, and other special seminar series through a combined, collaborative effort of one or more of the following: Columbia's Zuckerman Institute, the Center for Precision Psychiatry, the Department of Neuroscience, the Doctoral Program in Neurobiology and Behavior and the Columbia Translational Neuroscience Initiative, and with support from the Kavli Institute for Brain Science.
More information and a full schedule can be found here.