Evan Macosko,MD, PhD

Associate Professor of Psychiatry and Neurobiology

Harvard Medical School and Institute Member of the Broad Institute of MIT and Harvard

Spatial and single cell genomics for next generation Neuroscience

The advent of next generation sequencing ushered in a ten-year period of exuberant technology development, enabling the quantification of gene expression and epigenetic features within individual cells, and within intact tissue sections. In this seminar, I will outline our technological contributions, beginning with the development of Drop-seq, a method for high-throughput single cell analysis, followed by the development of Slide-seq, a technique for measuring genome-wide expression at 10 micron spatial resolution. Using a combination of these techniques, we recently constructed a comprehensive cell type atlas of the adult mouse brain, positioning cell types within individual brain structures. I will discuss the major findings from this dataset, including emerging principles of neurotransmission, and the localization of disease gene signatures to specific cell types. Finally, I will introduce a new spatial technology, Slide-tags, that unifies single cell and spatial genomics into a single, highly scalable assay.

Host(s): Andres Bendesky (Faculty) 

Please contact [email protected] with any questions.

This event will be in-person only and will not offer a Zoom option.
Open only to Columbia University and Columbia University Affiliates.
Speaker Location: Jerome L. Greene Science Center, 9th Floor Lecture Hall

Live-stream Location: CUIMC, Hammer Health Sciences Center, Lower Level 1, Room 107 

Tuesdays@10 is a signature Zuckerman Institute initiative that aims to expose researchers at all levels to high-quality science and stimulate scientific discourse. The speakers featured in this series represent various fields and techniques in neuroscience, and are either external to Columbia (Columbia Neuroscience Seminars and Special Seminars) or are Columbia faculty members (Local Circuits) invited through a combined, collaborative effort of one or more of the following: Columbia's Zuckerman Institute, the Department of Neuroscience, the Doctoral Program in Neurobiology and Behavior and the Columbia Translational Neuroscience Initiative, and with support from the Kavli Institute for Brain Science. 

More information and a full schedule can be found here.